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  • Title: Heme requirement for reproduction of a free-living nematode.
    Author: Hieb WF, Stokstad EL, Rothstein M.
    Journal: Science; 1970 Apr 03; 168(3927):143-4. PubMed ID: 5417058.
    Abstract:
    The free-living hermaphroditic nematode Caernorhabditis briggsae has a nutritional requirement for heme. The organism can be subcultured repeatedly in a chemically defined axenic medium that contains autoclaved bacterial cells (Escherichia coli) and sterols if a hemeprotein-containing fraction from liver is present. Pure myoglobin, hemoglobin, cytochrome c, and hemin, respectively, can substitute effectively for the liver fraction.
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