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  • Title: [Cytochemical studies in megakaryocytes in hematologic diseases].
    Author: Kolarz G, Ottmeier H, Singer F, Pietschmann H.
    Journal: Folia Haematol Int Mag Klin Morphol Blutforsch; 1975; 102(5):507-14. PubMed ID: 54299.
    Abstract:
    Bone marrow smears of 48 patients consisting of 12 normal cases, 36 patients with different haematological diseases-among them 9 cases of idiopathic thrombopenia, 4 cases of polycythaemia, and 9 cases of Hodgkin's disease - were examined cytochemically. Acid phosphatase, unspecific esterases, naphthol-AS-D-chloroacetate esterase, peroxydase, and leucin-aminopeptidase were represented; in addition the PAS reaction, fastgreen staining at pH 1.1, methyl-green pyronin staining and the lipid representation with Sudan black B were carried out. Besides those responses known from literature the different behaviour of acid megacaryocyte phosphatase in different haematological diseases must be particularly emphasized from all reactions.
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