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  • Title: Improvement in specificity of immunofluorescent reagents for identifying Streptococcus mutans by DEAE-cellulose-bacterial cell column immunosorption methods.
    Author: Mc Kinney RM, Thacker L.
    Journal: J Dent Res; 1976 Jan; 55():A50-7. PubMed ID: 54376.
    Abstract:
    We have described a new method for preparing bacterial cell columns, in which DEAE-cellulose is used as the cell support material. The columns can be used to isolate pure antibody or to remove cross-reacting antibodies from immune IgG. The columns are remarkable stable, can be scaled up to any desired dimensions, and should be suitable for large-scale production of highly specific serological reagents. The method is practical for obtaining serotype-specific and polyvalent IF reagents for identifying S. mutans organisms. However, the method could also be used as a tool for sophisticated studies of antigenic relationships that exist among these and other taxonomic groups of microorganisms.
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