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  • Title: Separation of polypeptide chains of ricin and the interaction of the A chain with Cibacron Blue F3GA.
    Author: Appukuttan PS, Bachhawat BK.
    Journal: Biochim Biophys Acta; 1979 Sep 29; 580(1):10-4. PubMed ID: 546433.
    Abstract:
    From ricin bound to the galactomannan guar gum in a column, the nonbinding toxic A chain could be eluted by reduction with 2-mercaptoethanol and later the B chain by lactose. The presence of a nucleotide-binding domain on the toxic chain A could be demonstrated from its interaction with the blue dye Cibacron Blue F3GA.
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