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  • Title: [Isolation and some properties of homogenous nuclease S1 from alpha-amyloryzin].
    Author: Senchenko VN, Kolbanovskaia EIu, Bocharov AL.
    Journal: Mol Biol (Mosk); 1979; 13(6):1377-83. PubMed ID: 547181.
    Abstract:
    A purification method for isolating homogeneous single-strand specific nuclease S1 from alpha-amyloryzin has been developed. The yield was about 16% and purification factor--9000. Nuclease S1 thus obtained was proved to be free of contaminations of any others nucleolytic enzymes. It is shown for the first time that ribo- and deoxy-dinucleosidemonophosphates are hydrolyzed by nuclease S1 to form 5'-nucleotides with pH optimum for ApA equal to 4.6.
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