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  • Title: The separation of nucleic acids and related compounds on nucleobase-coupled cellulose.
    Author: Ueda T, Miura K, Kobayashi S, Fujiwara T, Yoshida M.
    Journal: Nucleic Acids Symp Ser; 1979; (6):s169-72. PubMed ID: 547230.
    Abstract:
    The affinity chromatography on uracil-coupled cellulose was carried out for the separation of nucleosides, nucleotides and oligonucleotides. Adenine derivatives exhibited a high affinity to uracil-cellulose, and sequencial isomers of oligonucleotides containing adenine residue were resolved. Poly(A) was strongly bound to uracil-cellulose and recovered by the elution with 7M urea. This procedure was extended to the isolation of mRNA containing poly(A) sequences.
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