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  • Title: Salt extraction of soluble HL-A antigens.
    Author: Reisfeld RA, Pellegrino MA, Kahan BD.
    Journal: Science; 1971 Jun 11; 172(3988):1134-6. PubMed ID: 5574518.
    Abstract:
    Extraction of cultured human lymphoid cells with hypertonic salt solutions (3 molar potassium chloride) resulted in high recoveries of membrane-associated histocompatibility (HL-A) antigens in soluble form with potent activity and marked immunologic specificity. The active principle was purified by preparative acrylamide-gel electrophoresis. Application of the hypertonic salt extraction method is now yielding sufficient HL-A antigen to begin the elucidation of the molecular basis of transplantation individuality.
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