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  • Title: Fluorometric determination of histamine in tuna: development of method.
    Author: Staruszkiewicz WF, Waldron EM, Bond JF.
    Journal: J Assoc Off Anal Chem; 1977 Sep; 60(5):1125-30. PubMed ID: 561055.
    Abstract:
    Tuna extracts are treated with an anion exchange resin to remove interfering materials, histamine is derivatized with o-phthaladehyde, and the fluorescence of the resulting compound is measured fluorometrically. Replicate analyses of acceptable and decomposed tuna packed in oil or water agreed within 1 mg at a level of 10 mg/100 g and within 12 mg at a level of 100 mg/100 g. Recoveries of histamine added to fish were greater than 90 and greater than 83% at levels of 10 and 100 mg/100 g, respectively. The new method is more rapid and specific and is simpler than previous methods because no liquid-liquid extractions or chromatographic separations of histamine are required. The sensitivity of the method allows quantitation of less than 10 mg histamine/100 g sample. The accuracy and precision of the fluorometric method are comparable to those of the official AOAC colorimetric method.
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