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  • Title: Reconstitution of catecholamine-sensitive adenylate cyclase activity: interaction of components following cell-cell and membrane-cell fusion.
    Author: Schwarzmeier JD, Gilman AG.
    Journal: J Cyclic Nucleotide Res; 1977 Aug; 3(4):227-38. PubMed ID: 562358.
    Abstract:
    Heterokaryons formed by the fusion of adenylate cyclase-deficient S49 cells and beta-adrenergic receptor-deficient B82 cells in the presence of cycloheximide display a catecholamine-sensitive adenylate cyclase activity. Similar complementation can be observed when receptor-replete membranes from the cyclase-deficient cell are fused with intact B82 cells. Using the cell fusion technique it can be demonstrated that the uncoupled S49 cell variant has a functional beta-adrenergic receptor but that this variant and the adenylate cyclase-deficient variant are not complementary. Hypothetically, both clones may share a common defect in regulatory components of adenylate cyclase or both may lack a specific coupling factor.
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