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  • Title: Translation of endogenous message in embryonic chick erythroid cell polysomes in a cell-free protein-synthesizing system.
    Author: Zagris N.
    Journal: Biochem Genet; 1977 Oct; 15(9-10):825-32. PubMed ID: 563230.
    Abstract:
    Polysomes from 5.5-day and 6.5-day embryonic chick erythroid cells contain messenger RNA (mRNA) which can be translated into products in a cell-free protein-synthesizing system. The products of the cell-free system coelectrophoresed with carrier globin chains from the cells from which the polysomal pellet was isolated. Wheat germ S30 fraction increases by 1.5--2.0 fold [3H] leucine incorporation into trichloroacetic acid-precipitable material directed by the endogenous mRNA on chick erythroid cell polysomes. The wheat germ probably provides a specific factor or factors absent in a shortage in the incubation medium.
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