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  • Title: Relative contributions of alpha-, beta-, and omega-oxidative pathways to in vitro fatty acid oxidation in rat liver.
    Author: Antony GJ, Landau BR.
    Journal: J Lipid Res; 1968 Mar; 9(2):267-9. PubMed ID: 5640506.
    Abstract:
    A method is presented for estimating the relative contribution of alpha-, beta-, and omega-oxidation to the metabolism of fatty acids. The distribution of (14)C among the carbon atoms of liver glycogen-glucose is determined after liver slices have been incubated with fatty acids labeled at single points in the chain. When these results are compared with similar data obtained after an incubation with acetate-1-(14)C and acetate-2-(14)C, conclusions can be drawn about the contributions of alpha-, beta-, and omega-oxidation in palmitate, oleate, and stearate, and of oxidative cleavage of the double bond in oleate. Beta-Oxidation is overwhelmingly predominant under the conditions used.
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