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  • Title: Incorporation of a fatty acid containing a photosensitive group into lipids of cultured cardiac cells from chick embryo.
    Author: Franchi A, Ailhaud G.
    Journal: Biochimie; 1977; 59(10):813-7. PubMed ID: 564214.
    Abstract:
    Radioactive 12-(4-azido-2-nitrophenoxy)-stearic acid (NAP-stearate) was synthetized; it behaves as a competitive inhibitor of long-chain fatty acids for the entry into cultured cardiac cells from chick embryo. After uptake, [3H] NAP-stearate was incorporated by an energy-dependent process into neutral and polar lipids. Photoactivation as a function of time leads to a covalent labelling of the cells: up to 31 per cent of the radioactivity was recovered in the 105 000 g cell pellet, mainly in proteins. These experiments show that fatty acids containing photosensitive groups would potentially allow to localize the proteins involved in the binding and/or in the transport of fatty acids.
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