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  • Title: Reaction of nitrosoguanidine (N-methyl-N'-nitro-N-nitrosoguanidine) with tobacco mosaic virus and its RNA.
    Author: Singer B, Fraenkel-Conrat H, Greenberg J, Michelson AM.
    Journal: Science; 1968 Jun 14; 160(3833):1235-7. PubMed ID: 5648262.
    Abstract:
    "Nitrosoguanidine" (N-methyl-N'-nitro-N-nitrosoguanidine) acts on polynucleotides in neutral aqueous solution, methylating guanine in the 7-position, and, to a much lesser extent, adenine. In 67 percent dimethylformamide the effect on the purines decreased, and what seems to be 3-methyl-cytosine appeared. In formamide solution no base changes were detected. Nitrosoguanidine had little mutagenic effect on tobacco mosaic virus RNA in water; it had greater effect in dimethylformamide, and even greater in formamide; it was a strong mutagen only when it acted on intact tobacco mosaic virus particles. Thus, neither the methylation of guanine nor the cytosine modification represents the main mutagenic event.
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