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  • Title: Step-wise cross-linking of pig heart lactate dehydrogenase by a heterobifunctional reagent.
    Author: Kolkenbrock H, Kiltz HH, Trommer WE.
    Journal: Biochim Biophys Acta; 1978 Aug 21; 535(2):216-21. PubMed ID: 567067.
    Abstract:
    Cross-linking of the essential cysteine-165 of pig heart lactate dehydrogenase (L-lactate: NAD+ oxidoreductase, EC 1.1.1.27) with lysine-179 was achieved by stepwise reaction with N-(4-azidocarbonyl-3-hydroxy-phenyl)-maleimide. These two residues are connected by kind of a hydrophobic channel which suggests that the essential cysteine is not modified via the active center as previously assumed.
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