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  • Title: [Action of egg lysozyme on representatives of the family Micrococcaceae. Its action on Micrococcus].
    Author: Safonova TB, Afanas'eva TI, Kravchenko NA, Cherkasov IA, Sobolev VR.
    Journal: Antibiotiki; 1978 Sep; 23(9):797-802. PubMed ID: 567955.
    Abstract:
    The results of the study of the effect of various concentrations of egg lysozyme on M. luteus and M. varians using 2 methods, i.e. serial dilutions in agar and turbidimetric are presented. It was found that the MIC of lysozyme for M. luteus ranged within wide limits, from less than 0.0003 to 1 mg/ml. M. varians was stable to lysozyme. The MIC for all the strains was 8 mg/ml. The turbidimetric method provided determination of general regularities in changes of the optical density in all the strains of M. luteus under the effect of various concentrations of lysozyme. On the basis of these data it was possible to consider the method as the most deep means for determining the intraspecies similarities in the surface structures of Micrococcus as compared to the method of serial dilutions in agar. The dynamics of the changes in the optical density of the M. luteus suspension markedly differed from that of M. varians.
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