These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: New laboratory scale purification of beta-amanitin from American Amanita phalloides.
    Author: Yocum RR.
    Journal: Biochemistry; 1978 Sep 05; 17(18):3786-9. PubMed ID: 568002.
    Abstract:
    A modern laboratory scale method for purifying beta-amanitin from the mushroom Amanita phalloides is described. The purification involves solubilizing the toxin from dried mushrooms, removing the lipids by chloroform extraction, desalting on Amberlite XAD-4, adsorption chromatography on Sephadex LH-20 at acidic and neutral pH, and ion-exchange chromatography on QAE-Sephadex. The resulting beta-amanitin is homogeneous by thin-layer chromatography and is biologically active.
    [Abstract] [Full Text] [Related] [New Search]