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  • Title: Dissociation of human adult epidermal cells by disulfide-reducing agents and subsequent trypsinization.
    Author: Hentzer B, Kobayasi T.
    Journal: Acta Derm Venereol; 1976; 56(1):19-25. PubMed ID: 56852.
    Abstract:
    Primary cultures of epidermal cells from human adult skin were dissociated by dithiothreitol (DTT) with subsequent trypsinization. After treatment with disulfide reducing agents, the desmosomes were either destroyed or showed a homogeneous ultrastructure. Trypsin separated the homogeneous desmosomes, whereas cell membranes, organelles, and nuclei remained undamaged. Trypsin did not influence normal desmosomes. A suspension of human epidermal cells was recultivated in new flasks. Two types of cells grew in the cultures--polygonal and spindle-shaped. The polygonal cells were maintained for 30 to 60 days and grew slowly. The spindle-shaped cells were leucine-amino-peptidase negative, grew actively, and were subcultured.
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