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  • Title: Partial purification of transcriptionally active nucleosomes from trout testis cells.
    Author: Levy B, Dixon GH.
    Journal: Nucleic Acids Res; 1978 Nov; 5(11):4155-63. PubMed ID: 569293.
    Abstract:
    Mononucleosomes (MN1) enriched in structural non-histone proteins and transcribed DNA sequences were obtained by limited digestion of trout testis nuclei with micrococcal nuclease followed by selective solubilization in 0.1 M NaCl. These monosomes consist of the four inner histones plus stoichiometric amounts of the non-histone protein H6, of the HMG group, complexed with 140 base pairs of DNA. Hybridization experiments indicate that MN1 DNA is enriched in sequences complementary to cytoplasmic polyadenylated RNA.
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