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  • Title: Further investigations on the affinity chromatography of unlabeled and labeled cortisol-binding immunoglobulin.
    Author: Gijzen AH.
    Journal: Clin Chim Acta; 1976 Jan 02; 66(1):77-84. PubMed ID: 57014.
    Abstract:
    A specific method for the isolation of cortisol-binding immunoglobulin from rabbit antiserum by affinity chromatography on sepharose-coupled haptene (sepharose-cortisol) is described. This isolation technique has the advantage that no nonspecific gamma-globulin is present in the eluate of the column; previous absorption of anti-BSA-globulin can be omitted. The nature of the isolated immunoglobulin is determined by immuno-electrophoresis. The same isolation procedure can be used for the purification of radioactive antibody for radioimmunometric assay after labeling the immunoglobulin with iodine-125.
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