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  • Title: Production of cellulases by Trichoderma reesei QM 9414 in fed-batch and continuous-flow culture with cell recycle.
    Author: Ghose TK, Sahai V.
    Journal: Biotechnol Bioeng; 1979 Feb; 21(2):283-96. PubMed ID: 570860.
    Abstract:
    The scope in improving enzyme productivities from the cellulose fermentation process is examined in laboratory-scale fermentors. The maximum productivity (30 IU/liter hr) is attained in a continuous-culture process with cell recycle using modified medium containing 0.5% cellulose. Optimum dilution rate and recycle ratio are determined as 0.025 hr-1 and 1.2, respectively, for the process. The system is analyzed and steady-state equations for predicting enzyme protein concentrations in the fermentor are developed. In fed-batch cultures, slow addition of cellulose at high concentrations can improve enzyme productivity by as much as 33% over a batch process. The scope and results of using modified medium for cellulase production are also presented.
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