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  • Title: Analysis in vitro of tolerance.
    Author: Forbes IJ.
    Journal: Immunology; 1969 Jun; 16(6):699-706. PubMed ID: 5786940.
    Abstract:
    The generation in vitro of cells synthesizing haemolytic antibody to sheep erythrocytes (plaque forming cells—PFC) was shown to be due to the combined activity of two distinct populations of cells—macrophages and lymphocytes. Suspensions of cells from mice rendered tolerant to sheep erythrocytes by injection of a large dose of antigen and cyclophosphamide showed a greatly reduced capacity to generate PFC if tested within the first 17 days after treatment. This loss of capacity to mount an immune response in vitro was restored by suspensions of lymphocytes from spleens of normal mice. The lymphocytes from recently paralysed mice failed to generate PFC when incubated with preparations of normal macrophages. The macrophages from paralysed mice were as active as normal macrophages in generating PFC. Tolerance in this model is, therefore, associated with a loss of function in vitro of the lymphocytes in the spleens of such animals. Most spleens tested 3 weeks after induction of paralysis, when recovery in the intact animal is incomplete, had a normal capacity to generate PFC in vitro.
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