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  • Title: Vitamin B12-binders in human body fluids. I. Antigenic and physico-chemical characteristics.
    Author: Hurlimann J, Zuber C.
    Journal: Clin Exp Immunol; 1969 Jan; 4(1):125-40. PubMed ID: 5819225.
    Abstract:
    Three factors binding vitamin B12 are detected in human gastric juice by autoradiographs of immunoelectrophoresis. Binder 1 is present only in gastric juice and gastric mucosa extract and corresponds to intrinsic factor. Binder 2 is present also in other excretions such as bile, lactoserum, saliva, in serum and leucocyte extracts. Binder 3 present in saliva and gastric juice in lower quantity than binder 2, seems derived from this last one by attachment of sialic acid molecules. There is no immunological identity between intrinsic factor and the other binders without intrinsic factor activity. Binder 3 is resistant to proteolysis in vivo and in vitro. Binder 2 is modified by peptic digestion in vivo but not in vitro. Binder 1 is insensitive to proteolysis if saturated by vitamin B12, but loses its binding capacity and/or immunological properties if the digestion takes place before fixation of vitamin B12. The three binders seem to have the same affinity for vitamin B12 as judged by dialysis experiments, and the same molecular weight of 60,000 as judged by volume exclusion on Sephadex G-200.
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