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  • Title: [L-valine transport by the actinomycete Actinomyces species 26-115, the producer of actinomycin C].
    Author: Alekhova TA, Orlova TI, Silaev AB.
    Journal: Antibiotiki; 1976 Apr; 21(4):301-4. PubMed ID: 58579.
    Abstract:
    Transport of L-valine by Actinomyces species 26-115, an organism producing actinomycin C depended on L-valine concentration in the medium and temperature and required a source of intrinsic energy. Km for L-valine transport was 3.5.10(-6)--6.0.10(-6) M. It somewhat differed from experiment to experiment. The above system transported also other neutral amino acids. L-isoleucine was a competing inhibitor of L-valine transport. The transport of L-valine was stereospecific. The activity of the transport system was regulated by the intracellular content of L-valine. Probably because of this the amino acid transport depended on the culture age, so far as the level of free valine in the mycelium at various stages of development was different.
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