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  • Title: A simplified colorimetric micromethod for determination of serum cholesterol.
    Author: Bhandaru RR, Srinivasan SR, Pargaonkar PS, Berenson GS.
    Journal: Lipids; 1977 Dec; 12(12):1078-80. PubMed ID: 593071.
    Abstract:
    A simple and rapid micromethod is described for quantitation of cholesterol in 5-10 microliter of serum using uranyl acetate to deproteinize the serum and o-phthalaldehyde reagent to develop color. Independent analyses of serum samples by the present method and by two reference methods, Abell-Kendall and automated colorimetric method, indicated accuracy of the micromethod (r = 0.99). Use of capillary blood specimens makes this procedure applicable for screening infants and small children.
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