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  • Title: Specificity of antigenic fractions of tuberculin.
    Author: Tuboly S, Szent-Iványi T.
    Journal: Acta Microbiol Acad Sci Hung; 1976; 23(1):23-8. PubMed ID: 59518.
    Abstract:
    Purified protein derivative from Mycobacterium bovis was separated into 6 fractions by electrophoresis at 1500 V/40 mA in pH 4.2 acetic acid-pyridine buffer. Further purification of one of the fractions on Sephadex G 25 column and by acid hydrolysis yielded antigen "PS" eliciting tuberculin reaction only in animals vaccinated with M. bovis BCG but not in those sensitized with M. avium and some fast-growing mycobacteria. The specificity of antigen "PS" was confirmed in vitro: the antigen induced blastic transformation only in lymphocytes of guinea pigs sensitized with M. bovis BCG.
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