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  • Title: The fate of acetyl groups derived from glucose in the isolated perfused goat udder.
    Author: Hardwick DC.
    Journal: Biochem J; 1966 Apr; 99(1):228-31. PubMed ID: 5965339.
    Abstract:
    1. In the isolated perfused goat mammary gland, both glucose and acetate contribute 20-30% of carbon to citrate via acetyl-CoA. 2. Carbon dioxide does not contribute significantly to the formation of acetyl-CoA. 3. ATP citrate lyase, which, in rats, yields acetyl-CoA for fatty acid synthesis, is absent from or very low in high-speed supernatant fraction of ruminant tissue. 4. It is reasonable to infer that intramitochondrial acetyl-CoA, to which both glucose and acetate contribute, is separated from extramitochondrial acetyl-CoA to which glucose does not contribute and which is used for fatty acid synthesis.
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