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  • Title: Effect of light-adaptation on the photoreaction of bacteriorhodopsin from Halobacterium halobium.
    Author: Ohno K, Takeuchi Y, Yoshida M.
    Journal: Biochim Biophys Acta; 1977 Dec 23; 462(3):575-82. PubMed ID: 597494.
    Abstract:
    Light-induced formation of the 410 nm intermediate was investigated on dark-and light-adapted bacteriorhodopsin. The amplitude of the light-induced absorption increase at 410 nm of the light-adapted bacteriorhodopsin was twice as large as that of the dark-adapted bacteriorhodopsin. The amount of protons released from bacteriorhodopsin in response to illumination was also enhanced by light-adaptation. The degree of the enhancement was independent of the temperature in the dark-adaptation. The relation between these photochemical events and the isomeric configurations of retinal is discussed.
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