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Title: Artifact produced in disc electrophoresis by ammonium persulfate. Author: Brewer JM. Journal: Science; 1967 Apr 14; 156(3772):256-7. PubMed ID: 6021045. Abstract: Ammonium persulfate, a common polymerizing agent for acrylamide gels, can inactivate yeast enolase and produce increased electrophoretic heterogeneity during disc electrophoresis in gels containing 8M urea. The use of riboflavin and light for polymerization or thioglycolate for removal of the persulfate are feasible alternatives.[Abstract] [Full Text] [Related] [New Search]