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  • Title: Evidence for regulation of protein synthesis at the translation level in response to dietary alterations.
    Author: von der Decken A.
    Journal: J Cell Biol; 1967 Jun; 33(3):657-63. PubMed ID: 6036528.
    Abstract:
    Rats were maintained for several days on a protein-free diet which, approximately 14 hr before decapitation, was changed to a protein-rich diet. Microsomal subfractions of liver were obtained by sucrose density gradient centrifugation. The sedimentation patterns of the microsomal subfractions and of the polysomes were found to be unchanged after the alteration of the diet whereas the ability to incorporate amino acid was markedly enhanced. No significant differences in the RNA/protein ratio of the microsomal subfractions were observed. The changes in amino acid incorporation which were unrelated to concurrent changes in the amount of polysomes are discussed.
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