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  • Title: The isolation of prothrombin, Factor IX and Factor X from human Factor IX concentrates.
    Author: Modi GJ, Blajchman MA, Ofosu FA.
    Journal: Thromb Res; 1984 Dec 15; 36(6):537-47. PubMed ID: 6084874.
    Abstract:
    A relatively simple and reproducible procedure is described for the isolation of functionally and electrophoretically homogeneous prothrombin, factor IX and factor X from clinical Factor IX Concentrates. The procedure involves ammonium sulphate fractionation; then chromatography on DEAE-Sephadex A50, dextran sulphate-Sepharose and heparin-Sepharose. High recovery of all three procoagulants was obtained: 32% for factor IX; 32% for factor X; 29% for prothrombin.
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