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  • Title: Vectors with restriction-site banks. I. pJRD158, a 3903-bp plasmid containing 28 unique cloning sites.
    Author: Davison J, Heusterspreute M, Merchez M, Brunel F.
    Journal: Gene; 1984 Jun; 28(3):311-8. PubMed ID: 6086459.
    Abstract:
    A DNA fragment has been constructed that contains many unique cloning sites not present in currently used Escherichia coli plasmid cloning vehicles. Insertion of this fragment into a modified version of pBR322 results in an AmpRTetR vector (pJRD158) of 3903 bp containing 28 unique cloning sites, four "almost unique" cloning sites, and eight unassigned unique 6-bp palindromes. The plasmid has the additional advantages of very high copy number and altered incompatibility. The latter permits it to be stably maintained in the same host as pBR322.
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