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  • Title: A simple procedure for the isolation of highly purified lysosomes from normal rat liver.
    Author: Yamada H, Hayashi H, Natori Y.
    Journal: J Biochem; 1984 Apr; 95(4):1155-60. PubMed ID: 6086593.
    Abstract:
    A procedure for the isolation of highly purified lysosomes from normal rat liver is described. The method depends on the swelling of mitochondria when the postnuclear supernatant fraction is incubated with 1 mM Ca2+. The lysosomes can then be separated from the swollen mitochondria by Percoll density gradient centrifugation. The lysosomal fraction obtained by our method was enriched more than 120-fold in terms of the marker enzymes with a yield of 25%. The electron microscopic examination and the measurement of the activities of marker enzymes for various subcellular organelles indicated that our lysosomal preparation was essentially free from contamination by other organelles.
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