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  • Title: [Phospholipase C inhibitor from Streptoverticillium mycoheptinicum].
    Author: Iakovleva EP, Rozhanskaia TI, Levit ZhD, Selezneva AA, Miasnikova LG.
    Journal: Prikl Biokhim Mikrobiol; 1984; 20(3):349-54. PubMed ID: 6087312.
    Abstract:
    Streptoverticillium mycoheptinicum, a producer of the antifungal antibiotic mycoheptin, was found to produce an inhibitor of Clostridium perfringens phospholipase C. Dynamics of the enzyme accumulation in the culture liquid filtrate was studied. A technique was developed for purification of the inhibitor, which includes adsorption, selective precipitation, ultrafiltration, gel chromatography and isoelectric focusing. The inhibitor was found to be of the peptide nature with the molecular weight of 3500-4000 and to exist in two isoforms with the isoelectric points of 8.15 and 8.50.
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