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  • Title: Partial purification and characterization of the soluble phosphatidate phosphohydrolase of rat liver.
    Author: Butterwith SC, Hopewell R, Brindley DN.
    Journal: Biochem J; 1984 Jun 15; 220(3):825-33. PubMed ID: 6087797.
    Abstract:
    A method is described by which the Mg2+-stimulated phosphatidate phosphohydrolase can be purified from the soluble fraction of liver from ethanol-treated rats. The increase in specific activity was about 416-fold. This involved purification by adsorption on calcium phosphate, chromatography on DE-52 DEAE-cellulose, separation on Ultrogel AcA-34 and chromatography on CM-Sepharose 6B. The effects of phosphatidylcholine, phosphatidate and Mg2+, Mn2+ and Zn2+ on the activity are described. Inhibitor studies indicate that the phosphohydrolase contains functional thiol groups and arginine residues.
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