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  • Title: Cloning of the Bacillus subtilis spoIIA and spoV A loci in phage phi 105DI:1t.
    Author: Savva D, Mandelstam J.
    Journal: J Gen Microbiol; 1984 Aug; 130(8):2137-45. PubMed ID: 6088673.
    Abstract:
    A 6.95 kb HindIII-generated DNA fragment from Bacillus subtilis 168 was inserted into the DNA of phage phi 105DI:1t. The recombinant phage (phi 105DS1) contained DNA of 33.8 kb as compared with 35.2 kb for phi 105DI:1t and 39.2 kb for the wild-type phage. In the presence of helper phage, phi 105DS1 complemented both spoIIA and spoV A mutations in B. subtilis.
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