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  • Title: Expression of a biologically active fragment of human IgE epsilon chain in Escherichia coli.
    Author: Liu FT, Albrandt KA, Bry CG, Ishizaka T.
    Journal: Proc Natl Acad Sci U S A; 1984 Sep; 81(17):5369-73. PubMed ID: 6089199.
    Abstract:
    cDNA corresponding to human IgE heavy (epsilon) chain mRNA was cloned from human IgE-secreting myeloma U266 cells. Partial nucleotide sequence analysis demonstrated that the cloned cDNA contained the coding region for about two-thirds of the CH2 and all of the CH3 and CH4 domains as well as the 3'-untranslated region. This epsilon cDNA was inserted into expression vector pUC7 and expression of an epsilon-chain fragment in Escherichia coli was demonstrated by protein blot analysis using 125I-labeled goat anti-human IgE as probe. The expression product was purified on a column of goat anti-human IgE-conjugated Sepharose 4B and the polypeptide was found to retain binding activity to human basophils.
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