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Title: Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase. Author: Avila P, de la Cruz F, Ward E, Grinsted J. Journal: Mol Gen Genet; 1984; 195(1-2):288-93. PubMed ID: 6092854. Abstract: In the presence of the Tn21 transposase, plasmids that contain a single Tn21 inverted repeat sequence fuse efficiently with other plasmids. This reaction occurs in recA strains, is independent of the transposon-encoded resolution system, and results in insertions into different sites in the recipient plasmid. All fusion products studied contained at least one complete copy of the donor plasmid; most also contained some duplication of it as well. The data are consistent with processive models of transposition.[Abstract] [Full Text] [Related] [New Search]