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  • Title: Guanine nucleotides modulate cell surface cAMP-binding sites in membranes from Dictyostelium discoideum.
    Author: Van Haastert PJ.
    Journal: Biochem Biophys Res Commun; 1984 Oct 30; 124(2):597-604. PubMed ID: 6093807.
    Abstract:
    D.discoideum contains kinetically distinguishable cell surface cAMP binding sites. One class, S, is slowly dissociating and has high affinity for cAMP (Kd = 15 nM, t1/2 = 15 s). A second class is fast dissociating (t 1/2 about 1 s) and is composed of high affinity binding sites H (Kd approximately 60 nM), and low affinity binding sites L (Kd = approximately 450 nM) which interconvert during the binding reaction. Guanine nucleotides affect these three binding types in membranes prepared by shearing D.discoideum cells through Nucleopore filters. The affinity of S for cAMP is reduced by guanine nucleotides from 13 nM to 25 nM, and the number of S-sites is reduced about 50%. The number of fast dissociating sites is not altered by guanine nucleotides, but these sites are mainly in the low affinity state. Half-maximal effects are obtained at about 1 microM GTP, 2 microM GDP and 10 microM Gpp(NH)p(guanyl-5'-yl-imidodiphosphate); ATP and ADP are without effect up to 1 mM. These results indicate that D.discoideum cells have a functionally active guanine nucleotide binding protein involved in the transduction of extracellular cAMP signals via cell surface cAMP receptors.
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