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  • Title: Mechanism of transposition of bacteriophage Mu: polarity of the strand transfer reaction at the initiation of transposition.
    Author: Mizuuchi K.
    Journal: Cell; 1984 Dec; 39(2 Pt 1):395-404. PubMed ID: 6094017.
    Abstract:
    The distribution of newly synthesized DNA strands in the transposition products of bacteriophage Mu made in an in vitro system has been analyzed. The results support a model in which all Mu transpositions are initiated by a pair of strand transfer reactions that attach the 3' ends of Mu DNA to 5' protruding staggered ends of the target DNA. Joining of these ends produces a pair of structures similar to replication forks at the ends of the Mu DNA. Successful initiation of replication at either one or both ends, followed by a round of semiconservative replication, results in formation of a cointegrate structure. When the intermediate structure fails to replicate, breakage of the junctions between the Mu sequence and the vector sequence derived from the donor molecule can lead to a simple insert with a pair of gaps at the 5' ends of the Mu DNA. Evidence for a gap repair process that completes the simple insertion process has been obtained.
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