These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Interactions between thyreostimulin and PAF-acether on thyroid cell metabolism.
    Author: Haye B, Aublin JL, Champion S, Lambert B, Jacquemin C.
    Journal: Eur J Pharmacol; 1984 Sep 03; 104(1-2):125-31. PubMed ID: 6094205.
    Abstract:
    1-O-Alkyl, 2-acetyl sn-glycerylphosphorylcholine (platelet activating factor (PAF)-acether) originally described as a platelet activating factor, is effective on various parameters in different cells. It seemed interesting to us to test it on porcine thyroid cells cultured for 1 to 5 days in the absence (control cells) or in the presence of 0.1 mU/ml thyreostimulin (TSH cells). At concentrations ranging from 0.5 to 2.5 microM, PAF-acether inhibited significantly the accumulation of cyclic AMP resulting from a 5 min incubation of the cells with TSH (40 mU/ml) or forskolin (0.1 mM). PAF-acether alone did not affect basal cyclic AMP accumulation. The maximal inhibition was obtained on a 3 day culture and amounted to 40-50%. The inhibition was transient and vanished after a 30 min incubation. The effects of PAF-acether (0.5 microM) on phospholipid metabolism depended closely upon the physiological state of the cells and upon the age of the culture. When PAF-acether was incubated for 2 h with [32P]phosphate, it mimicked the effects of TSH, i.e. it increased phosphatidylinositol (PI) labelling on 1 day control cells (expected effect) and decreased it with 1 day TSH cells (reverse effects). The PAF-acether effect was rapid in onset. After cell prelabelling for 2 h in the presence of TSH, PAF-acether added for 15 min completely counteracted the hormone effects on PI and phosphatidylcholine (PC) but increased the phosphatidic acid (PA) labelling. The effect of PAF- acether on PI labelling was partially antagonized by forskolin.(ABSTRACT TRUNCATED AT 250 WORDS)
    [Abstract] [Full Text] [Related] [New Search]