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  • Title: Isolation and bacterial expression of a murine alpha leukocyte interferon gene.
    Author: Daugherty B, Martin-Zanca D, Kelder B, Collier K, Seamans TC, Hotta K, Pestka S.
    Journal: J Interferon Res; 1984; 4(4):635-43. PubMed ID: 6094686.
    Abstract:
    A murine alpha interferon gene (MuIFN-alpha A) was isolated from a murine genomic DNA library in phage lambda. The gene was cloned and its sequence determined. It was expressed in Escherichia coli under the control of the trp promoter/operator which resulted in antiviral activity on mouse L-cells. MuIFN-alpha A exhibits 60% homology to HuIFN-alpha A at the amino acid level.
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