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  • Title: A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures.
    Author: Dluzewski AR, Ling IT, Rangachari K, Bates PA, Wilson RJ.
    Journal: Trans R Soc Trop Med Hyg; 1984; 78(5):622-4. PubMed ID: 6095494.
    Abstract:
    Mature asexual parasites from cultures of knobby or knobless clones of P. falciparum containing 5 to 10% late forms were harvested by layering up to 10(9) erythrocytes on 3 ml Percoll. The density of the Percoll was adjusted to between 1.081 and 1.091 g cm-3, depending on the maturity of the parasites. Centrifugation at 1500 g for 10 min produced a sharp band at the interface containing mature parasites with a purity averaging 86%. Washed parasites derived from the layer or pellet showed good viability in vitro.
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