These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [A specific and quantitative determination of rat beta-endorphin. Combination of HPLC and RIA].
    Author: Iguchi Y, Tokuda H, Kishioka S, Ozaki M, Tamura S, Yamamoto H.
    Journal: Nihon Yakurigaku Zasshi; 1984 Oct; 84(4):385-93. PubMed ID: 6096234.
    Abstract:
    A specific and quantitative method for the determination of rat beta-endorphin by the combination of HPLC and RIA was developed. Rabbit antiserum against camel beta-endorphin (c beta-E) was raised and used for RIA at the final concentration of 1:10000. The quantitative range estimated from the displacement curve was 0.1-2.0 ng. Cross-reactivities with Met-Enk, Leu-Enk, alpha-MSH, alpha-endorphin, ACTH and human beta-E were less than 0.1, less than 0.1, less than 0.1, less than 0.1, 2 and 100%, respectively. These peptides were separated from each other by reversed phase HPLC with UV254 nm detection, and the minimum detectable dose of c beta-E was found to be 1 microgram. beta-E-like immunoreactivity (beta-ELIR) in the HPLC effluent was determined by RIA. The HPLC-RIA chromatogram of authentic c beta-E exhibited a single peak which coincided with the peak of c beta-E detected by UV, and 80% of the injected c beta-E (1-100 ng) was detected in the c beta-E fraction. The HPLC-RIA chromatogram of rat pituitary, hypothalamus, cerebrospinal fluid and plasma revealed the presence of 1-3 peaks, one of which was observed at the position of c beta-E. The HPLC elution of rat pituitary resolved the material into two peaks of biological activity, one of which coincided with the peak of beta-ELIR at the position of c beta-E. The HPLC-RIA chromatogram of the c beta-E fraction from pituitary obtained by gel-chromatography exhibited three peaks, one of which coincided with c beta-E. These results suggest that beta-ELIR in the c beta-E fraction of the HPLC elution may reflect rat beta-E accurately.
    [Abstract] [Full Text] [Related] [New Search]