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  • Title: Propagation of large quantities of poliovirus in human lymphoblastoid cells grown in a serum-free medium.
    Author: Bjare U.
    Journal: J Virol Methods; 1984 Nov; 9(3):259-68. PubMed ID: 6097596.
    Abstract:
    All three types of poliovirus replicated to good titers in Epstein-Barr virus-transformed human lymphoblastoid cells isolated from patients with infectious mononucleosis. Cells were grown in a serum-free medium, which consisted of enriched Eagle's medium with addition of 0.1% albumin, 0.1% Intralipid and 1 mg/l of transferrin. Large quantities of virus could be produced in 2- to 20-l roller flasks or in spinner flasks. The propagated poliovirus gave an antibody response that complies with requirements for vaccine production.
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