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  • Title: Bovine serum albumin: characterization of a fatty acid binding site on the N-terminal peptic fragment using a new spin-label.
    Author: Hsia JC, Wong LT, Tan CT, Er SS, Kharouba S, Balaskas E, Tinker DO, Feldhoff RC.
    Journal: Biochemistry; 1984 Dec 04; 23(25):5930-2. PubMed ID: 6098304.
    Abstract:
    A new spin-label, 4-(L-glutamo)-4'-[(1-oxy-2,2,5,5-tetramethyl-3L-pyrrolidinyl )amino]-3, 3'-dinitrodiphenyl sulfone, is shown to bind to one high-affinity binding site on bovine serum albumin (K = 5 X 10(4) M-1, n = 1). Analysis of the binding of the spin-label to the amino-terminal half (peptic fragment PB) and the carboxy-terminal half (peptic fragment PA) of BSA, and their complex (PA-PB), indicates that the spin-label binds to a long-chain fatty acid binding site located on PB. The usefulness of the novel specificity of the spin-label in characterizing this binding site is discussed.
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