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  • Title: Cloning of a third nitrate reductase gene from the cyanobacterium Anacystis nidulans R2 using a shuttle cosmid library.
    Author: Kuhlemeier CJ, Teeuwsen VJ, Janssen MJ, van Arkel GA.
    Journal: Gene; 1984 Nov; 31(1-3):109-16. PubMed ID: 6098517.
    Abstract:
    A strategy for gene cloning in the cyanobacterium Anacystis nidulans R2 was developed which made use of a gene library constructed in a shuttle cosmid vector. The method involved phenotypic complementation of mutants with pooled cosmid DNA. The development of the procedure and its application to the cloning of a third gene involved in nitrate reduction are described.
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