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  • Title: Physical characterization of the Clostridium perfringens tetracycline-chloramphenicol resistance plasmid pIP401.
    Author: Magot M.
    Journal: Ann Microbiol (Paris); 1984; 135B(3):269-82. PubMed ID: 6099703.
    Abstract:
    Four restriction endonucleases were used to construct a physical map of the tetracycline-chloramphenicol resistance plasmid pIP401, which had been isolated from Clostridium perfringens. Twenty-seven restriction sites were placed within the 52-kb map, including 1 site for AvaI, 2 sites for KpnI, 10 sites for EcoRI and 14 sites for PstI. The loss of chloramphenicol resistance in the derived pIP406 plasmid was associated with a deletion of a 6.2-kb DNA segment located in a 10.55 EcoRI-PstI fragment. Two 1.4-kb inverted repeats were also characterized in both pIP401 and pIP406.
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