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  • Title: Incorporation of ammonium in amino acids by Trypanosoma cruzi.
    Author: Caldas RA, Araújo EF, Felix CR, Roitman I.
    Journal: J Parasitol; 1980 Apr; 66(2):213-6. PubMed ID: 6104692.
    Abstract:
    Ammonium ions were incorporated into L-glutamate and alpha-ketoglutarate in epimastigote forms of Trypanosoma cruzi through the following enzymatic systems: NADPH and NADH-dependent glutamate dehydrogenase, NADPH-dependent glutamate synthase, L-glutamine synthetase and NADH-dependent glutamate synthase in order of decreasing specific activity (mumoles of product formed/min/mg protein). The pH optima and Km's for the glutamate dehydrogenase system were determined. Disc electrophoresis showed the presence of cathodic bands of GDH activity, which were highly dependent on NADP+.
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