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  • Title: Physical and functional mapping of rat-liver mitochondrial DNA.
    Author: Kazakova TB, Babich SG, Mel'nikova MP, Tzimbalenko NV, Braga EA, Nosikov VV.
    Journal: Mol Cell Biochem; 1981 Feb 26; 35(1):39-47. PubMed ID: 6111746.
    Abstract:
    Rat-liver mitochondrial DNA (mtDNA) contains 2 cleavage sites of the restriction endonuclease Xbal. The molecular sizes of restriction fragments are 6.6 X 10(6) and 3.7 X 10(6) D. The results of partial cleavage of mtDNA with EcoRI allow the fragment F (0.32 X 10(6) D) to be localized in the sequence ABCEGFHDA. The functional map of mtDNA is constructed for two genes of the ATP-ase mRNAs from rat-liver mitochondria. Molecular hybridization shows that the ATPase genes are located in fragment B and in the GEHD area of mtDNA EcoRI cleavage.
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