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  • Title: [Spectrofluorometric topography of the ATPase center of Ca2+-Mg2+-dependent ATPase of sarcoplasmic reticulum by means of platinum and palladium compounds].
    Author: Tat'ianenko LV, Kotel'nikova RA, Moshkovskiĭ IuSh, Zakharova IA, Bankovskiĭ IuA.
    Journal: Mol Biol (Mosk); 1981; 15(2):424-9. PubMed ID: 6113541.
    Abstract:
    The fluorescence of tryptophan residues in Ca2+--Mg2+-ATPase was studied in the presence of K2PtCl4, K2PdCl4 and 5-sulpho-8-mercaptochinolinate platinum and palladium. It has been shown that both first two compounds quenched the fluorescence dye to bonding with SH-groups in ATPase active centre, but the last two compounds influence the fluorescence by bonding with tryptophan residues. The distance between the SH-groups and tryptophan in the active centre was determined by Foerster--Galanin equation and was equal to 14 +/- 3 A.
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